The first production aircraft (pictured above) completed its first flight in April 1940, and production ended in December 1940. The production aircraft were designated He 116 A-0 and not He 116 B-0, as incorrectly stated in many publications, and initially all had civil registrations, later they received military codes (StKz - Stammkennzeichen or VKz - Verbandskennzeichen). As the He 116 A-0 was unarmed it was operated over Germany and occupied territories only, but never near the frontline.
The type served first in the Versuchstelle für Höhenflüge (test center for high-altitude flights) and with reconnaissance groups of the supreme commander of the Luftwaffe, later they were deregulated to LdKdo (Luftdienstkommandos, air service commands) and schools. In January 1944, only three were still in the inventory of the Luftwaffe, two of them in schools."